Physical assessment, Hematological and Serum Amyloid A levels Pre and Post exercise in Arabian Horses in Maiduguri and Jere, Borno State. Nigeria
Physical Assessment, Hematological and Serum Amyloid A Levels Pre and Post Exercise in Arabian Horses
Keywords:
Exercise, hematology, serum amyloid A, Arabian horsesAbstract
Long-duration activities, such as endurance races, cause significant changes in hematological and biochemical components as a result of central and peripheral exhaustion. The aim of the present study was to assess the physical health status as well as hematological and serum amyloid A (SAA) levels pre- and post-galloping exercise in Arabian horses in Maiduguri and Jere, Borno State, Nigeria. Before and after the galloping exercise, the health status of 50 healthy Arabian horses was physically assessed and blood samples were collected for hematological and serum biochemistry analyses. Packed cell volume (PCV), red blood cell (RBC), haemoglobin (Hb) concentrations, white blood cell (WBC), and differential leucocytes such as neutrophils, eosinophils, basophils, monocytes, and lymphocytes were determined using ballotment methods and haematocrit cyanmethaemoglobin. SAA levels pre- and post-galloping were determined using a commercial enzyme-linked immunosorbent assay (ELISA) kit. The physical parameters such as temperature, heart, and respiratory rates values were significantly increased at p < 0.001, 0.001, 0.050, and 0.001, respectively, after the galloping exercise as compared with the pre-galloping values. Haematological parameters such as PCV, RBC, and Hb were increased significantly at p < 0.001, 0.001, and 0.015, respectively, as well as eosinophils and monocytes at p < 0.040 and 0.001, respectively, after galloping as compared with the pre-galloping values. The mean values of SAA increased significantly (p < 0.007) post-galloping. Therefore, it was concluded that the galloping type of exercise affects some physical parameters such as temperature, respiration, and heart rates as well as various hematological parameters and biochemical parameters such as SAA, and an increase in SAA could be due to resistance provided by the sandy terrain, leading to exhaustion in exercising the muscles of horses in the study area.
References
Argent G. Inked: Human-Horse Apprenticeship, Tattoos, and Time in the Pazyryk World. Society & Animals 2013;21:178–93. https://doi.org/10.1163/15685306-12341301.
Birke L, Hockenhull J. Journeys Together: Horses and Humans in Partnership. Society & Animals 2015;23:81–100. https://doi.org/10.1163/15685306-12341361.
McGreevy PD, Griffiths MD, Ascione FR, Wilson B. Flogging tired horses: Who wants whipping and who would walk away if whipping horses were withheld? PLOS ONE 2018;13:e0192843. https://doi.org/10.1371/journal.pone.0192843.
Alexandra B. The concept of violence’; A proposed framework for the study of animal protection law and policy. PhD. Australian National University, 2016.
Fan YK, Hsu JC, Peh HC, Tsang CL, Cheng SP, Chiu SC, et al. The Effects of Endurance Training on the Hemogram of the Horse. Asian-Australasian Journal of Animal Sciences 2002;15:1348–53.
Rezazadeh F, Hosseinzadeh N, Jozani RJ, Dianati N. Comparison of Some Hematological Parameters Between Horses in an Endurance Competition. Animal and Veterinary Sciences 2017;4:97. https://doi.org/10.11648/j.avs.20160406.13.
FAOSTAT. Global Equine population update 2014.
Mikkelsen K, Stojanovska L, Polenakovic M, Bosevski M, Apostolopoulos V. Exercise and mental health. Maturitas 2017;106:48–56. https://doi.org/10.1016/j.maturitas.2017.09.003.
Thompson W. Worldwide Survey Reveals Fitness Trends for 2009. ACSM’s Health & Fitness Journal 2008;12:7–14. https://doi.org/10.1249/01.FIT.0000312432.13689.a4.
Cywińska A, Szarska E, Górecka R, Witkowski L, Hecold M, Bereznowski A, et al. Acute phase protein concentrations after limited distance and long distance endurance rides in horses. Res Vet Sci 2012;93:1402–6. https://doi.org/10.1016/j.rvsc.2012.02.008.
Adamu L, Adzahan NM, Abdullah R, Ahmad B. Effects of Race Distance on Physical, Hematological and Biochemical Parameters of Endurance Horses. American Journal of Animal and Veterinary Sciences 2010;5:244–8. https://doi.org/10.3844/ajavsp.2010.244.248.
Teixeira-Neto AR, Ferraz GC, Moscardini ARC, Albernaz RM, Gondin MR, Queiroz-Neto A. Do hematologic constituents really increase due to endurance exercise in horses? Pesq Vet Bras 2012;32:951–6. https://doi.org/10.1590/S0100-736X2012000900023.
Bhatti R, Din M, Shaikh A. The Effect of Exercise on Blood Parameters. Pak J Physiol 2007;3.
Adamu L, Mohd Adzahan N, Abdullah R, Fateh Mohamed BA. Effect of strenuous sub-maximal race on heart rates of endurance horses. Malaysian Journal of Veterinary Research 2012;3:29–32.
Jain S, Gautam V, Naseem S. Acute-phase proteins: As diagnostic tool. J Pharm Bioallied Sci 2011;3:118–27. https://doi.org/10.4103/0975-7406.76489.
Csilla T, Oskar N, Kovac G. Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Veterinární Medicína 2014;59:163–80. https://doi.org/10.17221/7478-VETMED.
Witkowska-Piłaszewicz O, Bąska P, Czopowicz M, Żmigrodzka M, Szczepaniak J, Szarska E, et al. Changes in Serum Amyloid A (SAA) Concentration in Arabian Endurance Horses During First Training Season. Animals (Basel) 2019;9:E330. https://doi.org/10.3390/ani9060330.
Witkowska-Piłaszewicz OD, Żmigrodzka M, Winnicka A, Miśkiewicz A, Strzelec K, Cywińska A. Serum amyloid A in equine health and disease. Equine Vet J 2019;51:293–8. https://doi.org/10.1111/evj.13062.
Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 2004;168:28–40. https://doi.org/10.1016/S1090-0233(03)00119-9.
Talat R. Infection of Haematozoan Parasites Found in Birds of NWFP (Pakistan) 2005;8:1–5. https://doi.org/10.3923/pjbs.2005.1.5.
Coles E, editor. Urinalysis and Laboratory Procedures. vol. 5. 4th ed. Philadelphia: W.B. Saunders Company; 1980.
AERC. Rider’s Handbook. Veterinary guidelines for judging AERC endurance competitions. Auburn, CA: 2006.
Fédération Equestre Internationale. Dressage Handbook Guidelines for Judging. Lausanne, Switzerland: Fédération Equestre Internationale; 2007.
Barnes A, Kingston J, Beetson S, Kuiper C. Endurance veterinarians detect physiologically compromised horses in a 160 km ride. Equine Vet J Suppl 2010:6–11. https://doi.org/10.1111/j.2042-3306.2010.00225.x.
Adamu L, Noraniza MA, Rasedee A, Bashir A. Effect of Age and Performance on Physical, Hematological, and Biochemical Parameters in Endurance Horses. Journal of Equine Veterinary Science 2013;33:415–20. https://doi.org/10.1016/j.jevs.2012.07.015.
Adamu L, Adzahan NM, Rasedee A, Ahmad B. Physical Parameters and Risk Factors Associated with the Elimination of Arabian and Crossed Arabian Endurance Horses during a 120-km Endurance Race. Journal of Equine Veterinary Science 2014;34:494–9. https://doi.org/10.1016/j.jevs.2013.10.175.
Kang O-D, Park Y-S. Effect of age on heart rate, blood lactate concentration, packed cell volume and hemoglobin to exercise in Jeju crossbreed horses. J Anim Sci Technol 2017;59:2. https://doi.org/10.1186/s40781-017-0126-8.
Malinowski K, Shock EJ, Rochelle P, Kearns CF, Guirnalda PD, McKeever KH. Plasma beta-endorphin, cortisol and immune responses to acute exercise are altered by age and exercise training in horses. Equine Vet J Suppl 2006:267–73. https://doi.org/10.1111/j.2042-3306.2006.tb05551.x.
Pedersen BK, Toft AD. Effects of exercise on lymphocytes and cytokines. British Journal of Sports Medicine 2000;34:246–51. https://doi.org/10.1136/bjsm.34.4.246.
Cuniberti B, Badino P, Odore R, Girardi C, Re G. Effects induced by exercise on lymphocyte β-adrenergic receptors and plasma catecholamine levels in performance horses. Res Vet Sci 2012;92:116–20. https://doi.org/10.1016/j.rvsc.2010.11.002.
Fallon KE. The acute phase response and exercise: the ultramarathon as prototype exercise. Clin J Sport Med 2001;11:38–43. https://doi.org/10.1097/00042752-200101000-00007.
Satué K, Hernández A, Muñoz A. Physiological Factors in the Interpretation of Equine Hematological Profile. In: Lawrie C, editor. Hematology - Science and Practice, IntechOpen; 2012. https://doi.org/10.5772/38961.
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Copyright (c) 2022 Dr. Yakaka Wakil, Dr. Lawan Adamu, Dr. Isa Gulani, Prof. Muhammad Modu Bukar
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