Haptoglobin, Magnesium, Adiponectin, and Leptin as Biomarkers for Obesity and Insulin Dysregulation in Horses
Relation of Biomarkers with Obesity and Insulin in Horses
Keywords:
horse, obesity, adiponectin, leptin, haptoglobin, insulin dysregulationAbstract
The aim of this project was to identify blood analytes which are associated with obesity and/or insulin dysregulation (ID) in horses. For this study, 57 horses were selected. At the start, body condition score (BCS) was established, and an oral sugar test (OST) was performed. Furthermore, the following blood analytes were determined: basal glucose, insulin, albumin, high molecular weight (HMW) adiponectin, leptin, haptoglobin, total protein, calcium, magnesium, phosphate, non-esterified fatty acids, and also glucose and insulin 75 min after an OST. Horses were classified according to their body condition as ‘lean’ (BCS 4/9-6/9) or ‘obese’ (BCS > 6/9). Horses were classified as insulin dysregulated (ID-positive) when insulin was 40 mU/L or higher than 75 min after OST. Multivariable logistic regression analysis showed a correlation between obesity and HMW adiponectin, leptin, total protein, and magnesium and between ID, haptoglobin, and leptin. Receiver operating characteristic plots (ROC) of obesity with leptin, adiponectin, total protein, and magnesium, which had an area under the curve (AUC) of 0.932, showed that the combination of these four blood analytes has a high association with obesity. The AUC of the ROC analysis of ID with haptoglobin and leptin was 0.805 implicating that this combination has a high association with ID. Although obesity can be observed (eyes and hands), the health risk of obesity can be monitored with a combination of leptin, adiponectin, total protein, and magnesium, while leptin and haptoglobin are good markers for ID (a health risk of obesity).
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Copyright (c) 2022 Dr. Guillaume Counotte, Prof. Andy Durham, Dr. Veerle Vandendriessche, Dr. Linda van den Wollenberg
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