Detection of Mycoplasma equigenitalium from Genital Tract of Healthy Domestic Donkeys (Equus africanus asinus)

Mycoplasma equigenitalium in Donkeys

Authors

  • Pablo Tamiozzo Department of Animal Pathology, Faculty of Agronomy and Veterinary Medicine, National University of Río Cuarto, Argentina
  • Erika Sticotti Department of Animal Pathology, Faculty of Agronomy and Veterinary Medicine, National University of Río Cuarto, Argentina
  • Ana Flores Bragulat Equine Production Laboratory, Department of Animal Production, Faculty of Agronomy and Veterinary Medicine, National University of Río Cuarto, Argentina
  • Carolina Alonso Equine Production Laboratory, Department of Animal Production, Faculty of Agronomy and Veterinary Medicine, National University of Río Cuarto, Argentina
  • Mauro Mació Department of Animal Pathology, Faculty of Agronomy and Veterinary Medicine, National University of Río Cuarto, Argentina
  • Luis Losinno Equine Production Laboratory, Department of Animal Production, Faculty of Agronomy and Veterinary Medicine, National University of Río Cuarto, Argentina

Keywords:

Mycoplasma equigenitalium, reproductive tract, vagina, prepuce, jennies, jacks

Abstract

Mycoplasma equigenitalium (M. equigenitalium) has been identified in the reproductive tracts of both fertile and infertile mares and stallions. However, there are scarce antecedents on its detection in the reproductive tract of donkeys. A cross-sectional study was conducted with healthy domestic donkeys. Jennies (n=6) and jacks (n=6) were sampled through the collection of vaginal and preputial swabs. The specimens were plated into a commercial Mycoplasma medium and analyzed through the use of a species-specific PCR. M. equigenitalium was detected by PCR in four specimens (4/12), one from a jack (1/6) and three from jennies (3/6). Moreover, from one of the PCR positive jennies, M. equigenitalium could be isolated. Presumably, this is the first report of M. equigenitalium detection from healthy domestic donkeys by both culture and PCR methods despite the small number of analyzed specimens. The obtained results are a starting point for the study of Mycoplasmas affecting donkeys.

References

Vallely LM, Egli-Gany D, Wand H, Pomat WS, Homer CSE, Guy R, et al. Adverse pregnancy and neonatal outcomes associated with Neisseria gonorrhoeae: systematic review and meta-analysis. Sex Transm Infect 2021;97:104–11. https://doi.org/10.1136/sextrans-2020-054653.

Tamiozzo PJ. Mycoplasma maculosum and Mycoplasma spumans associated with fertility disorders in dogs from a Bernese Mountain dog kennel. Rev Argent Microbiol 2022;54:39–42. https://doi.org/10.1016/j.ram.2021.04.001.

Moorthy AR, Spradbrow PB, Eisler ME. Isolation of mycoplasmas from the genital tract of horses. Aust Vet J 1977;53:167–9. https://doi.org/10.1111/j.1751-0813.1977.tb00158.x.

Kirchhoff H. Mycoplasma equigenitalium, a new species from the cervix region of mares. International Journal of Systematic Bacteriology (USA) 1978;28:496–502.

Heitmann J, Kirchhoff H, Petzoldt K, Sonnenschein B. Isolation of acholeplasmas and mycoplasmas from aborted horse fetuses. Vet Rec 1979;104:350. https://doi.org/10.1136/vr.104.15.350-a.

Kirchhoff H, Heitmann J, Bisping W. [Mycoplasmas isolated from the genital tract of mares (author’s transl)]. Zentralbl Bakteriol A 1980;246:228–35.

Bermudez V, Miller R, Johnson W, Rosendal S, Ruhnke L. Recovery of Mycoplasma spp. from the Reproductive Tract of the Mare during the Estrous Cycle. Can Vet J 1987;28:519–22.

Spergser J, Aurich C, Aurich JE, Rosengarten R. High prevalence of mycoplasmas in the genital tract of asymptomatic stallions in Austria. Vet Microbiol 2002;87:119–29. https://doi.org/10.1016/s0378-1135(02)00043-3.

Nehra K, Rana R, Viswas KN, Arun TR, Singh VP, Singh AP, et al. Isolation and molecular identification of Mycoplasma equigenitalium from equine genital tracts in northern India. Iran J Vet Res 2015;16:176–81.

Hui Q, Baoxiang Z, Wenke W. Studies on mycoplasma from genital tracts donkeys I.Isolation and Identification of Acholeplasma Iaidlawii. Gansu Nongye Daxue Xuebao 2002;37:338–41.

Pearson RA, Ouassat M. Estimation of the liveweight and body condition of working donkeys in Morocco. Vet Rec 1996;138:229–33. https://doi.org/10.1136/vr.138.10.229.

Pearson RA, Ouassat M. A Guide to Body Condition Scoring and Live Weight Estimation of Donkeys. Centre for Tropical Veterinary Medicine. Scotland: Centre for Tropical Veterinary Medicine, University of Edinburgh; 2000.

Câmara RJF, Bueno BL, Resende CF, Balasuriya UBR, Sakamoto SM, dos Reis JKP. Viral Diseases that Affect Donkeys and Mules. Animals (Basel) 2020;10:2203. https://doi.org/10.3390/ani10122203.

Thiemann AK, Bell NJ. The Peculiarities of Donkey Respiratory Disease. In: Lekeux P, editor. Equine Respiratory Diseases, United States: International Veterinary Information Service; 2014.

Burden F, Thiemann A. Donkeys Are Different. Journal of Equine Veterinary Science 2015;35:376–82. https://doi.org/10.1016/j.jevs.2015.03.005.

Barrandeguy ME, Carossino M. Infectious Diseases in Donkeys and Mules: An Overview and Update. Journal of Equine Veterinary Science 2018;65:98–105. https://doi.org/10.1016/j.jevs.2018.02.026.

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Published

2022-09-15

How to Cite

Tamiozzo, P. ., Sticotti, E. ., Flores Bragulat, A. ., Alonso, C. ., Mació, M. ., & Losinno, L. . (2022). Detection of Mycoplasma equigenitalium from Genital Tract of Healthy Domestic Donkeys (Equus africanus asinus): Mycoplasma equigenitalium in Donkeys. International Journal of Equine Science, 1(2), 33–35. Retrieved from https://rasayely-journals.com/index.php/ijes/article/view/28

Issue

Vol. 1 No. 2 (2022)

Section

Original Articles

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Copyright (c) 2022 Pablo Tamiozzo, Erika Sticotti, Ana Flores Bragulat, Carolina Alonso, Mauro Mació, Luis Losinno

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