Taking the Bitter with the Sweet - A Preliminary Study of the Short-Term Response of Horses to Various Tastants in Solutions
Keywords:
taste perception, preference test, water intake, sour tasteAbstract
Horses can distinguish sweet, salty, sour, and bitter tastes, but little is known about their preferences for various tastants. Understanding horse taste preferences can aid in increasing water intake by adding a preferred tastant or by masking an unpleasant taste to encourage administration of medications, for example. The quantity of water intake by horses was examined over five separate trials involving a two-choice preference test between tap water and water containing varying concentrations of sucrose (0-50g/100ml), citric acid (0-2.43mg/100ml), quinine (0-30mg/100ml) or a mix of sucrose (10mg/100ml)/citric acid (1.31mg/100ml) and sucrose (10mg/100ml)/quinine (20mg/100ml). Horses (n = 5) showed a weak preference for sweetened water up to 10mg/100ml (p < .001), with a rejection at higher concentrations. Horses rejected all concentrations of both sour (n = 12 horses; p < .001) and bitter (n = 6 horses; p < .001) solutions. In the mixed tastant trials, sucrose mixed with citric acid was only weakly rejected compared to the sucrose solution alone, which was moderately rejected (n = 5 horses; p < .001). Similarly, mixed sucrose/quinine solution intake increased over the quinine solution alone (n = 9 horses; p < .001). There was a large variation among individual horses within each trial, with some horses strongly rejecting sucrose solutions and others strongly preferring citric acid solutions. No horse indicated a preference for bitter solution in any trial. Age (p < .001), breed (p < .001), and exercise (p = .004) all influenced total fluid intake in the sour trial, not dependent on treatment (p = .063). These preliminary results show that some horses appear to prefer sweet and a preferred tastant can mask a less preferred tastant.
References
Beaver BV. Chapter 2 - Equine behavior of sensory and neural origin. In: Beaver BV, editor. Equine Behavioral Medicine, Academic Press; 2019, p. 31–64. https://doi.org/10.1016/B978-0-12-812106-1.00002-4.
Lunceford BE, Kubanek J. Reception of aversive taste. Integrative and Comparative Biology 2015;55:507–17. https://doi.org/10.1093/icb/icv058.
Gilbertson TA. The physiology of vertebrate taste reception. Current Opinion in Neurobiology 1993;3:532–9. https://doi.org/10.1016/0959-4388(93)90052-z.
Lindemann B. Receptors and transduction in taste. Nature 2001;413:219–25. https://doi.org/10.1038/35093032.
Molero-Chamizo A, Rivera-Urbina GN. Taste processing: Insights from animal models. Molecules 2020;25:3112. https://doi.org/10.3390/molecules25143112.
Palmer RK. A pharmacological perspective on the study of taste. Pharmacological Reviews 2018;71:20–48. https://doi.org/10.1124/pr.118.015974.
Liu WW, Bohórquez DV. The neural basis of sugar preference. Nature Reviews Neuroscience 2022;23:584–95. https://doi.org/10.1038/s41583-022-00613-5.
London RM, Snowdon CT, Smithana JM. Early experience with sour and bitter solutions increases subsequent ingestion. Physiology & Behavior 1979;22:1149–55. https://doi.org/10.1016/0031-9384(79)90270-1.
Christensen CM, Brand JG, Malamud D. Salivary changes in solution pH: a source of individual differences in sour taste perception. Physiology & Behavior 1987;40:221–7. https://doi.org/10.1016/0031-9384(87)90211-3.
Denton DA. The Hunger for Salt: An Anthropological, Physiological, and Medical Analysis. Springer-Verlag; 1982.
Kyriazakis I, Tolkamp BJ, Emmans G. Diet selection and animal state: an integrative framework. Proceedings of the Nutrition Society 1999;58:765–72. https://doi.org/10.1017/s0029665199001044.
Goatcher WD, Church DC. Taste responses in ruminants. III. reactions of pygmy goats, normal goats, sheep and cattle to sucrose and sodium chloride. Journal of Animal Science 1970;31:364–72. https://doi.org/10.2527/jas1970.312364x.
Urata K, Manda M, Watanabe S. Behavioral study on taste responses of hens and female Japanese quails to salty, sour, sweet, bitter and umami solutions. Nihon Chikusan Gakkaiho 1992;63:325–31. https://doi.org/10.2508/chikusan.63.325.
Takayama K, Mizoguchi Y, Oshima I, Nakanishi Y. Behavioral study on taste responses of geese to sweet, salty, sour, bitter, and umami solutions. Animal Behaviour and Management 2018;54:68–74. https://doi.org/10.20652/abm.54.2_68.
Randall RP, Schurg WA, Church DC. Response of horses to sweet, salty, sour and bitter solutions. Journal of Animal Science 1978;47:51–5. https://doi.org/10.2527/jas1978.47151x.
Goatcher WD, Church DC. Taste responses in ruminants. I. Reactions of sheep to sugars, saccharin, ethanol and salts. Journal of Animal Science 1970;30:777–83. https://doi.org/10.2527/jas1970.305777x.
Goatcher WD, Church DC. Taste responses in ruminants. II. reactions of sheep to acids, quinine, urea and sodium hydroxide. Journal of Animal Science 1970;30:784–90. https://doi.org/10.2527/jas1970.305784x.
Goatcher WD, Church DC. Taste responses in ruminants. IV. Reactions of pygmy goats, normal goats, sheep and cattle to acetic acid and quinine hydrochloride. Journal of Animal Science 1970;31:373–82. https://doi.org/10.2527/jas1970.312373x.
Van Diest TJ, Kogan CJ, Kopper JJ. The effect of water flavor on voluntary water intake in hospitalized horses. Journal of Equine Veterinary Science 2021;98:103361. https://doi.org/10.1016/j.jevs.2020.103361.
Lindinger MI. Oral electrolyte and water supplementation in horses. Veterinary Sciences 2022;9:626. https://doi.org/10.3390/vetsci9110626.
Francis JM, Neander CR, Roeder MJ, Perry EB. The influence of topically applied oil–based Palatants on eating behavior in horses. Journal of Equine Veterinary Science 2020;91:102995. https://doi.org/10.1016/j.jevs.2020.102995.
Thorne JB, Goodwin D, Kennedy MJ, Davidson HPB, Harris P. Foraging enrichment for individually housed horses: Practicality and effects on behaviour. Applied Animal Behaviour Science 2005;94:149–64. https://doi.org/10.1016/j.applanim.2005.02.002.
Houpt KA, Zahorik DM, Swartzman-Andert JA. Taste aversion learning in horses. Journal of Animal Science 1990;68:2340. https://doi.org/10.2527/1990.6882340x.
Thomas LC, Wright TC, Formusiak A, Cant JP, Osborne VR. Use of flavored drinking water in calves and lactating dairy cattle. Journal of Dairy Science 2007;90:3831–7. https://doi.org/10.3168/jds.2007-0085.
van den Berg M, Giagos V, Lee C, Brown WY, Cawdell-Smith AJ, Hinch GN. The influence of odour, taste and nutrients on feeding behaviour and food preferences in horses. Applied Animal Behaviour Science 2016;184:41–50. https://doi.org/10.1016/j.applanim.2016.08.015.
Nombekela SW, Murphy MR, Gonyou HW, Marden JI. Dietary preferences in early lactation cows as affected by primary tastes and some common feed flavors. Journal of Dairy Science 1994;77:2393–9. https://doi.org/10.3168/jds.s0022-0302(94)77182-4.
Enke N, Brinkmann L, Südekum K, Tholen E, Gerken M. Sensitivity of ponies to sodium in the drinking water. Animal Science Journal 2022;93. https://doi.org/10.1111/asj.13697.
Manda M, Urata K, Noguchi T, Watanabe S. Behavioral study on taste responses of cattle to salty, sour, sweet, bitter, umami and alcohol solutions. Nihon Chikusan Gakkaiho 1994;65:362–7. https://doi.org/10.2508/chikusan.65.362.
Duncan IJH. Measuring preferences and the strength of preferences. Poultry Science 1992;71:658–63. https://doi.org/10.3382/ps.0710658.
O’Mahony M, Wichchukit S. The evolution of paired preference tests from forced choice to the use of ‘No Preference’ options, from preference frequencies to d′ values, from placebo pairs to signal detection. Trends in Food Science & Technology 2017;66:146–52. https://doi.org/10.1016/j.tifs.2017.05.017.
Cheon E, Reister EJ, Hunter SR, Mattes RD. Finding the sweet spot: measurement, modification, and application of sweet Hedonics in humans. Advances in Nutrition 2021;12:2358–71. https://doi.org/10.1093/advances/nmab055.
Sterk A, Schlegel P, Mul AJ, Ubbink-Blanksma M, Bruininx EMAM. Effects of sweeteners on individual feed intake characteristics and performance in group-housed weanling pigs1. Journal of Animal Science 2008;86:2990–7. https://doi.org/10.2527/jas.2007-0591.
Jankunis ES, Whishaw IQ. Sucrose bobs and quinine Gapes: horse (Equus caballus) responses to taste support phylogenetic similarity in taste reactivity. Behavioural Brain Research 2013;256:284–90. https://doi.org/10.1016/j.bbr.2013.08.024.
Janczarek I, Wilk I, Pietrzak S, Liss M, Tkaczyk S. Taste preferences of horses in relation to their breed and sex. Journal of Equine Veterinary Science 2018;64:59–64. https://doi.org/10.1016/j.jevs.2018.02.010.
Miyamoto T, Fujiyama R, Okada Y, Sato T. Acid and salt responses in mouse taste cells. Progress in Neurobiology 2000;62:135–57. https://doi.org/10.1016/s0301-0082(99)00072-6.
Dziezak JD. Acids: natural acids and acidulants. In: Caballero B, Finglas PM, Toldrá F, editors. Encyclopedia of Food and Health, Oxford: Academic Press; 2016, p. 15–8. https://doi.org/10.1016/B978-0-12-384947-2.00004-0.
Valentová H, Panovská Z. Sensory evaluation | taste. In: Caballero B, editor. Encyclopedia of Food Sciences and Nutrition (Second Edition), Oxford: Academic Press; 2003, p. 5180–7. https://doi.org/10.1016/B0-12-227055-X/01069-5.
Murphy K, Wishart S, Mills D. The acceptability of various flavoured solutions by Thoroughbred horses. Equine Veterinary Journal 1999;31:67.
van den Berg M, Giagos V, Lee C, Brown WY, Hinch GN. Acceptance of novel food by horses: The influence of food cues and nutrient composition. Applied Animal Behaviour Science 2016;183:59–67. https://doi.org/10.1016/j.applanim.2016.07.005.
Kristula MA, McDonnell SM. Drinking water temperature affects consumption of water during cold weather in ponies. Applied Animal Behaviour Science 1994;41:155–60. https://doi.org/10.1016/0168-1591(94)90020-5.
McDonnell SM, Kristula MA. No effect of drinking water temperature (ambient vs. chilled) on consumption of water during hot summer weather in ponies. Applied Animal Behaviour Science 1996;49:159–63. https://doi.org/10.1016/0168-1591(96)01041-6.
Mars LA, Kiesling HE, Ross TT, Armstrong JB, Murray L. Water acceptance and intake in horses under shipping stress. Journal of Equine Veterinary Science 1992;12:17–20. https://doi.org/10.1016/s0737-0806(06)81378-x.
McCutcheon LJ, Geor RJ. Sweating: Fluid and ion losses and replacement. Veterinary Clinics of North America: Equine Practice 1998;14:75–95. https://doi.org/10.1016/s0749-0739(17)30213-4.
Watson PJ, Swartwood JN. Responsivity to water regulatory challenges in rats adapted to fluids adulterated with quinine or citric acid. Physiology & Behavior 1990;47:789–93. https://doi.org/10.1016/0031-9384(90)90098-o.
Cappai MG, Aboling S. Chapter 9 - Toxic or harmful components of aromatic plants in animal nutrition. In: Florou-Paneri P, Christaki E, Giannenas I, editors. Feed Additives, Academic Press; 2020, p. 147–58. https://doi.org/10.1016/B978-0-12-814700-9.00009-1.
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